Skip to main content
. Author manuscript; available in PMC: 2009 Apr 15.
Published in final edited form as: Biochem Pharmacol. 2008 Jan 20;75(8):1610–1615. doi: 10.1016/j.bcp.2008.01.006

Figure 3. Effect of AZT on thymidine phosphorylation in cultured cells.

Figure 3

Flasks of cells were incubated in growth media with unlabeled AZT (0–200 μM) and a total [methyl-3H]-thymidine concentration of 0.12 μM in U-937 and Raji (1550 DPM/pmol) and 1.4 μM in H9c2 (4000 DPM/pmol). Cellular extracts were prepared after 10 min of incubation as described in Materials and Methods. Data represents the mean ± SEM of three to four trials, shown as the percent of the 0 μM AZT control versus AZT concentration. Total TNP represents the sum of TMP, TDP, and TTP. Both TTP and TMP were detected in the U-937 (left) and Raji (center) trials. However, TTP was the only phosphorylated form of thymidine seen with the H9c2 cells (right), so only the Total TNP line is shown. The 50% inhibitory concentration (IC50) for AZT inhibition of total thymidine phosphorylation was calculated from the best fit line (dashed lines) (SigmaPlot 10).