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. 2008 May 14;3(5):e2162. doi: 10.1371/journal.pone.0002162

Figure 4. Immunoblotting analysis of epithelial-mesenchymal transition markers in stable cells expressing AQP5 and mutants.

Figure 4

Expression of epithelial proteins including E-cadherin, α-catenin and γ-catenin, and mesenchymal proteins including fibronectin and vimentin was examined by immunoblotting in the four separate BEAS-2B cells, each of which carries overexpression construct for AQP5 (clone #1 and #2), N185D, S156A and Mock. Only cells with AQP5 exhibited a loss of epithelial markers and a gain of mesenchymal cell markers. 1, AQP5 clone#1; 2, AQP5 clone#2; 3, N185D; 4, S156A; 5, Mock based on BEAS-2B cell lines.