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. 2008 May 14;3(5):e2178. doi: 10.1371/journal.pone.0002178

Figure 1. SipA and SptP are co-expressed in S. Typhimurium.

Figure 1

(A) SipA and SptP expression in wt S. Typhimurium. M1269 was grown under TTSS-1 inducing conditions, immobilized on gelatine-coated coverslips, fixed, permeabilized and SptP and SipA pools in the bacterial cytosol were immuno-strained (Materials and Methods). DNA was stained with DAPI. (B) Fraction of bacteria harboring SipA (open bars), SptP (black bars) and fraction of the SipA+ bacteria also harboring SptP (grey bars); Data was obtained from experiments as described in (A) and (C); n>400 bacteria, two independent experiments; (C) SipA and SptP expression in hilA over-expressing S. Typhimurium. M1269 (pHilA) was grown and analyzed as described in (A). (D) FACS-analysis of pHilA-induced sipA-expression. M2001 harbors the transcriptional reporter tsr-venus integrated downstream of the chromosomal sipA gene. FACS analysis of Tsr-Venus fluorescence is shown for M2001 and M2001 (pHilA). Wt S. Typhimurium (w/o tsr-venus reporter) served as a negative control. The gate is indicated as a box harboring 1.1% of wt control bacteria, 19.6% of M2001 and 90.8% of M2001(pHilA).