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. 2008 May 5;181(3):497–510. doi: 10.1083/jcb.200712064

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© 2008 Hara et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 6. — FAK is not required for autophagy. (A) FAK^+/− and FAK^−/− MEFs were cultured in the complete or starvation medium for the indicated times with or without 100 nM bafilomycin A₁. The cell lysates were subjected to immunoblot analysis with the indicated antibodies. (B) FAK^+/− and FAK^−/− MEFs stably expressing GFP-LC3 were cultured in complete or starvation medium for 120 min. The cells were fixed and examined by fluorescence microscopy. (C) Wild-type MEFs stably expressing GFP-FAK were cultured in complete or starvation medium for 120 min. The cells were fixed and subjected to immunofluorescence microscopy using anti-Atg16 antibody. Black squares indicate the enlarged areas shown in insets. Bars, 20 μm.