Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 May 5;181(3):421–429. doi: 10.1083/jcb.200711053

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2008 Goshima et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 4. — Characterization of human Dgt6 in HeLa cells. (A) Alignment of N-terminal regions of Dgt6 proteins from D. melanogaster, Xenopus laevis, and Homo sapiens. Identical amino acids are boxed and the similar ones are hatched. (B) Uniform spindle localization of GFP-tagged human Dgt6 in metaphase (green). (C) Dim γ-tubulin and sparse MT phenotypes after RNAi knockdown of hDgt6 in HeLa cells (72 h). (D) Quantitation of γ-tubulin or MT level at centrosomes and spindles. Ratio of spindle and centrosome signal intensity in hDgt6 RNAi cells (γ-tubulin, 0.17 ± 0.02 SEM [n = 14]; MT, 0.46 ± 0.02 SEM [n = 19]) was significantly (P < 0.0005) lower than in control (γ-tubulin, 0.33 ± 0.04 SEM [n = 7]; MT, 0.75 ± 0.02 SEM [n = 15]). γ-tubulin (red) and DNA (blue) were counterstained. Bars, 10 μm.