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. 2008 May 5;181(3):537–549. doi: 10.1083/jcb.200707022

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© 2008 Kakinuma et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 1. — Kank interacts with 14-3-3 isoforms γ, ε, η, and θ. (A) Kank interacts with 14-3-3θ. VMRC-RCW cells were lysed and the lysate was immnunoprecipitated (IP) with control rabbit IgG (lane 1) or anti-Kank pAb (lane 2). The immunoprecipitates and the lysate (lane 3) were subjected to Western blotting (WB) using the antibodies indicated. (B) Endogenous Kank and 14-3-3 proteins are colocalized in VMRC-RCW cells. A fluorescence image of Kank (green, lane 1), a fluorescence image of 14-3-3 (red, lane 2), a merged image of lanes 1 and 2 (yellow, lane 3), and a light microscope image (lane 4) are shown. Bar, 10 μm. (C) Kank associates with 14-3-3 isoforms γ, ε, η, and θ. HEK293T cells were transfected with the indicated expression vectors. Immnunoprecipitation was performed using an anti-FLAG antibody and the bound proteins were detected by Western blotting using the antibodies indicated.