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. 2008 May 5;181(3):447–460. doi: 10.1083/jcb.200710028

Figure 5.

Figure 5.

Effects of TIN2 mutants in normal cells. (a) TIN2-15C suppresses proliferation of normal cells. We infected presenescent HCA2 cells with lentiviruses expressing GFP, wild-type (wt) TIN2, TIN2-13, or TIN2-15C and determined the cell number the indicated number of days thereafter. Plotted are cumulative cell numbers versus days in culture. (b) TIN2-15C induces cellular senescence. HCA2 cells were infected with lenti-GFP or lenti-TIN2-15C and assessed 10 d later for senescence-associated β-galactosidase as described previously (Dimri et al., 1995). Replicatively senescent HCA2 cells were used as positive controls. Bars, ∼10 μm. (c) TIN2-13 and TIN2-15C induce damage foci in presenescent and senescent cells. Presenescent (P) and senescent (S) HCA2 cells were infected with lenti-TIN2-13 or lenti-TIN2-15C and stained 48 h later for expression of the mutant proteins and γH2AX or 53BP1 foci. 50–80 cells were scored in two or three independent experiments for each transfection. Error bars represent the standard deviation.