Table I. Telomeric fusions caused by expression of TIN2 mutants.
Lentiviral vector | No. metaphases analyzed | No. chromosome fusions
|
Fusions per metaphase | Fusions per chromosome | |
---|---|---|---|---|---|
+Tel | –Tel | ||||
GFP | 53 | 0 | 0 | 0 | 0 |
TIN2-13 | 53 | 7 | 3 | 0.18 | 0.0034 |
TIN2-15C | 61 | 42 | 10 | 0.85 | 0.0175 |
Presenescent HCA2 cells were infected with a retroviruses expressing GSE-22, selected, and infected with lentiviruses expressing GFP, TIN2-13, or TIN2-15C. The cells were treated with colcemid, and telomeres on metaphase chromosomes were identified by PNA-FISH as described in Materials and methods. Chromosome fusions were scored by fluorescence in situ hybridization for the presence (+Tel) or absence (−Tel) of telomeric DNA at the site of fusion.