Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 May 5;181(3):411–419. doi: 10.1083/jcb.200802189

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2008 Kim et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 3. — Full-length CENP-E is a slow plus end–directed kinetochore motor. (A) Coomassie-stained full-length CENP-E–GFP (366 kD) purified from baculovirus-induced insect cells. (B) Microtubule gliding assay with full-length CENP-E. CENP-E–GFP proteins were tethered to a GFP antibody-coated surface of a flow chamber, and polarity-marked microtubules were subsequently introduced. Minus ends of the microtubules are brightly marked. Colored arrowheads indicate the starting positions of three microtubules, and colored dots indicate the minus ends. The mean gliding velocity was 30 ± 7.6 nm/s (mean ± SD; n = 112). (C) Purified full-length CENP-E–GFP was added into the Xenopus extract before spindle assembly. (D) Added CENP-E–GFP was localized to the kinetochore in Xenopus extract spindle. A frame from a time-lapse video of a metaphase spindle in the Xenopus extracts is shown. Red, X-rhodamine tubulin; green, CENP-E–GFP. Bars: (B) 2 μm; (D) 10 μm.