Table 1.

Primers and PCR Amplification Conditions to Sequence the Coding Region of Human POLG2^a

Exon	Primer Forward	Primer Reverse	Temperature, °C	Size, bp
1	TGAGTGATGGGAGAGTGTGC	TCCGACTACTTCAAAAAGATGAGAA	62	488
2–3	CCACCAAGCTTAGCCAACAT	GCCCAACAAATGTTTTTACCA	63	680
4	TCGCACATTTGCTGAATAAAA	GACACCACGTTTGCACCTTA	60	381
5	GGCCAGGTGACAGAGTGAGA	TTCCTGTGGCCAGATTCTAAA	63	391
6	GGGGGCAGCTGAATATGTTA	CGAGATCCAAAATGGTCCTG	62	357
7	AGGGTGATTTGTGGCTTCAC	TCCCTGCTGAGGCAATTAAC	62	360
8	TGAGTATTCTCTTCACAGTTTTGGTT	AAGGCAAAGGGGCTAGAAAT	62	332

Abbreviations: bp, base pair; PCR, polymerase chain reaction; POLG2, gene encoding the accessory subunit (p55) of polymerase γ.

^aThe PCR amplification conditions were as follows: first cycle, 96°C for 3 minutes; second cycle, 35 cycles of 94°C for 35 seconds, melting temperature for 35 seconds, and 72°C for 35 seconds; and last cycle, 72°C for 10 minutes.