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. 2004 Jun 8;91(1):178–185. doi: 10.1038/sj.bjc.6601902

Figure 3.

Figure 3

E2 inhibition of resveratrol-induced activation of MAPK and p53 is time dependent. MCF-7 cells were treated with E2 (10−9 M) alone for 4 h or for different time periods (0–4 h) with resveratrol (RV, 10 μM, 4 h), after which nuclear fractions were prepared from each sample. This series of immunoblots, representative of three experiments, indicates that minimal activation of ERK1/2 by resveratrol (top panel, lane 3) was enhanced by 1–2 h incubation with E2 (lanes 4 and 5). A longer incubation with E2 caused a time-dependent reduction in ERK1/2 phosphorylation (lanes 4–7, P<0.05). Resveratrol induced phosphorylation of serines 15, 20 and 392 of p53 (pSer15-, pSer20- and pSer392-p53), and acetylation of p53 (lane 3 in each panel). However, these effects of resveratrol on p53 post-translational modification were progressively inhibited by coincubation with E2 for 1–4 h. The reductions in resveratrol-induced serine-15, serine-20, and serine-392 phosphorylation and acetylation of p53 by E2 were significant at P<0.003 or less. Molecular weight markers in this figure are not shown, but are similar to those shown in Figure 1.