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. 2004 Jun 8;91(1):178–185. doi: 10.1038/sj.bjc.6601902

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Copyright © 2004 Cancer Research UK

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E₂ inhibits p53-inducible p21 expression and apoptosis caused by resveratrol. (A) MCF-7 cells were treated with 10 μM resveratrol in the presence or absence of 10⁻⁹ M E₂ for 24 h. Based on measurement of p21 and GAPDH cDNA band densities (upper and lower images, respectively), and correction of p21 densities for the levels of GAPDH, a 2.5-fold increase in p21 cDNA abundance was seen with resveratrol treatment (lane 3 compared with lane 1). This increase in p21 expression was significant (P<0.025) by analysis of variance. E₂, 10⁻⁹ M, blocked p21 transcription induced by resveratrol (lane 4 compared with lane 3, P<0.05), but had no effect in the absence of resveratrol. (B) MCF-7 cells were treated with 10 μM resveratrol in the presence or absence of 10⁻⁹ M E₂ for 24 h. Resveratrol caused DNA fragmentation, indicating apoptosis as shown in this representative figure (lane 3). This effect of resveratrol was inhibited by E₂ (lane 4).