Table 2. Percentage Philadelphia chromosome positive colonies (CFU-GM) in patients with CML in the presence or absence of 8Cl-cAMP.
|
Week 0 |
Week 5 |
|||||
|---|---|---|---|---|---|---|
| Patient ID | Control | 8Cl-cAMP 50 μm | 8Cl-cAMP 100 μm | Control | 8Cl-cAMP 50 μm | 8Cl-cAMP 100 μm |
| 1216 (cp) | 100 (2/2) | 100 (20/20) | 100 (28/28) | ND | 14 (3/21) | 0 (0/15) |
| 1206 (cp) | 100 (12/12) | ND | 70 (12/17) | 83 (30/36) | ND | 0 (0/15) |
| 1726 (cp) | 100 (62/62) | ND | 100 (25/25) | 83 (26/29) | ND | 0 (0/37) |
| 166 (D) | 100 (6/6) | 100 (15/15) | ND | 100 (20/20) | 46 (7/15) | ND |
| A97/3 (ap) | 100 (21/21) | ND | 100 (30/30) | 100 (5/5) | ND | 83 (5/6) |
| 371 (ap) | 81 (27/33) | ND | 92 (24/26) | 81 (27/33) | ND | 33 (1/3) |
| 361 (ap) | 100 (30/30) | ND | 100 (19/19) | 100 (30/30) | ND | 50 (2/4) |
| 341 (ap) | 100 (40/40) | ND | 96 (26/27) | 100 (34/34) | ND | 62 (5/8) |
The Ph1 chromosome positive colonies (in %) arising from patient bone marrow cells in percent are summarised, the actual number of colonies is given in parenthesis (Ph1 chromosome+/total number). MNC were incubated for 24 h without or with 50 or 100 μm of 8-Cl-cAMP as indicated, washed and resuspended in long-term culture medium (Dexter). CFU-assays were setup in week 0 and weekly thereafter. CFU-GM of the adherent fraction of the cells (ADC) is shown, since there were only few colonies in the non-adherent fraction at this time. Ph1 chromosome status was determined by cytogenetic analysis. cp=chronic phase; D=diagnosis; ap=accelerated phase; ND=not determined.