Figure 2.

Effect of bisphosphonates on the induction of apoptosis in melanoma cells. Preconfluent A375 (A) and M186 (B) melanoma cells were treated with the indicated concentrations of zoledronate (white columns), or pamidronate (grey columns) for 24 h. (C) A375 (light grey columns) and M186 (hatched columns) cells were treated for 24 h with clodronate in the indicated concentrations. DNA fragmentation was measured using the ‘Cell death detection ELISA^PLUS’ as described under Materials and methods. Four independent experiments were performed in quadruplicate, with similar results. One representative experiment is shown. Results are given as % of controls±s.d. (n=4) (^*P<0.05; ^**P<0.01).