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. 2004 Jul 27;91(4):803–810. doi: 10.1038/sj.bjc.6602052

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Copyright © 2004 Cancer Research UK

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Nitrogen-containing bisphosphonates induce caspase-3/7 activity in melanoma cells. Preconfluent A 375 (A) and M186 (B) cells were treated with vehicle control (white bars), 100 μM pamidronate (grey columns) or 100 μM zoledronate (black columns) for 24 h. For specific activation of caspase-3, cells were pretreated with the respective caspase-3 inhibitor 1 h prior to stimulation and then treated for 24 h with 100 μM pamidronate or zoledronate in combination with the inhibitor (square bars). Caspase-3/7 activity was determined with the Apo-One™ homogeneous caspase-3/7 assay as described under Materials and methods. Three independent experiments were performed in quadruplicate, with similar results. One representative experiment is shown. Results are given as % of control±s.d. (n=4) (^*P<0.05; ^**P<0.01).