Table 2. Sensitivity, specificity and predictive values of the Mcm5 test for oesophageal cancer.
| Test | % Sensitivity (95% CI) | % Specificity (95% CI) | % PPV (95% CI) | % NPV (95% CI) |
|---|---|---|---|---|
| Mcm5 test ⩾1500 cut point | 95 (75–99) | 48 (29–68) | 58 (39–75) | 93 (66–99) |
| Mcm5 test ⩾5000 cut point | 85 (62–97) | 85 (66–96) | 81 (58–95) | 88 (70–98) |
| Mcm5 test ⩾7500 cut point | 75 (51–91) | 96 (81–99) | 94 (70–99) | 84 (66–95) |
Mcm5=minichromosome maintenance protein 5; CI=confidence interval; PPV=positive predictive value; NPV=negative predictive value.
a
Three cut-off points were used to demonstrate test performance under different circumstances as follows: at the lower detection limit of the assay (fluorescence signal generated from 1500 proliferating HeLa S3 cells well−1), where sensitivity of the test was maximal; at the point where the false-positive and false-negative rates of the test were equal (5000 cells well−1); and where specificity exceeded 95% (7500 cells well−1).