Fig. 7.

SK&F 96365 does not affect Ca²⁺_O-mediated influx. As in Fig. 1, incubation of DRG neurons in 50 nM free Ca²⁺_O did not decrease [Ca²⁺]_i. After incubating DRG neurons in 50 nM free Ca²⁺_O for 60 min, neurons were incubated in 10 μM SK&F 96365 for 5 min prior to re-addition of 1.8 mM Ca²⁺_O. Incubation in drug modestly increased Ca²⁺_i above resting levels. Fluorescence values are the mean ± S.E.M. of 13 cells. Inset: larger time resolution demonstrates that SK&F 96365 increased Ca²⁺_i. Note that the increase in Ca²⁺_i in the presence of drug (○) was indistinguishable from control (●).