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. Author manuscript; available in PMC: 2008 May 2.
Published in final edited form as: J Biol Chem. 2007 Oct 20;283(1):445–452. doi: 10.1074/jbc.M703910200

Figure 2. Actin cross-linking by ACD is accompanied by the release of inorganic phosphate.

Figure 2

Inorganic phosphate (Pi) release during the course of cross-linking of 10 μM actin was measured with the EnzChek Phosphate kit (Invitrogen). When added to 10 μM Mg-G-actin, KabC (closed black circles) blocks actin polymerization as well as the subsequent ATP hydrolysis and Pi release - which normally accompany actin polymerization (open black circles). Addition of LFNACD to non-polymerizable actin-KabC complex at 1 to 200 (cyan), 1 to 500 (magenta), and 1 to 1000 (green) mole ratios causes dose- and time-dependent release of Pi. LFNACD alone does not produce any noticeable amount of Pi under conditions used here (blue).