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. Author manuscript; available in PMC: 2008 May 2.
Published in final edited form as: J Biol Chem. 2007 Oct 20;283(1):445–452. doi: 10.1074/jbc.M703910200

Figure 5. The efficiency of the cross-linking depends on Mg2+ concentration.

Figure 5

Actin-KabC (10 μM) complex was cross-linked by LFNACD (1 to 500 mole ratio) for 20 minutes in the presence of 200 μM ATP and increasing concentrations of MgCl2. The amount of actin cross-linked was analyzed as in Fig. 3B, normalized to 100%, and plotted as a function of free MgCl2 concentration in the solution (calculated by WinMAXC software, Version 2.40, Stanford University). Solid line represents single ligand binding fitting, with an apparent Kd = 0.54 mM.