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. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: J Immunol. 2008 Mar 1;180(5):3103–3112. doi: 10.4049/jimmunol.180.5.3103

Figure 8. Regulatory dendritic cells (RDC) can regulate donor-reactive DTH responses.

Figure 8

Dendritic cells (DC) isolated from late (day 150) renal allograft acceptor splenocytes were combined with alloreactive splenocytes and donor antigen alone or alloantigen + polyclonal antibodies to IDO. These combinations were injected into pinnae of naïve C57BL/6 mice for trans-vivo DTH measurements. Addition of donor antigen to allosplenocytes caused significant swelling (*p=0.02). RDCs regulated responses of alloreactive splenocytes to donor antigen (**p=0.001), and blocking secreted indoleamine 2, 3 dioxygenase (IDO) restored alloreactivity (***p=0.003). DCs from normal mice did not regulate alloreactive splenocytes with or without IDO antibody (data not shown). DTH responses were measured after 24 hours as change in ear thickness (mean ± SD x 10-4 inches).