Figure 2.

Synchronized cell assay for cell-cycle delay. Synchronization was achieved with hydroxyurea and α-factor in a–c, and with α-factor alone in d. a, d, Percentage of mitoses that produce binucleate large-budded cells and binucleate unbudded cells against time following release from arrest. a, One representative experiment. Two independent experiments gave similar results. b, c, Viability (b) and cell number (c) for these and additional control strains are plotted against time. Table 1 shows extra data from experiments in which hydroxyurea was used. Wt, wild type; ts, temperature sensitive.