Table 1.

Cell-cycle-delay assay

		Unbudded cells			Budded cells
Time after hydroxyurea release (min)	Strain	No nucleus	One nucleus per cell	Two nuclei	Both nuclei in mother	One nucleus in mother, one in bud	One nucleus in mother, none in bud
0	WT	0	11	0	1	1	87
	act5-ts	0	10	0	0	0	88
	yeb1Δ	0	9	0	0	0	91
	yeb1Δact5-ts	0.5	8	1	2	1	87
	yeb1-29act5-ts	0.5	8	1.5	2.5	1.5	81
30	WT	0	14	0	0	7	79
	act5-ts	0	13	0	4	5	78
	yeb1Δ	0	11	0.5	0.5	6	81
	yeb1Δact5-ts	0.5	11	1	3	5	79
	yeb1-29act5-ts	1	9	1	2	6	81
60	WT	0	15	0	1	26	58
	act5-ts	0	15	0	8	23	54
	yeb1Δ	0	18	1	1	20	60
	yeb1Δact5-ts	1	17	3	8	19	52
	yeb1-29act5-ts	3	14	4	9	21	49
75	WT	0	36	0	1	45	18
	act5-ts	0	32	0	14	35	19
	yeb1Δ	0	34	1	1	41	23
	yeb1Δact5-ts	8	21	11	8	34	18
	yeb1-29act5-ts	9	22	12	7	35	15
90	WT	0	66	1	1	24	8
	act5-ts	0	44	1	19	25	11
	yeb1Δ	0	59	1	1	26	13
	yeb1Δact5-ts	7	39	15	7	23	9
	yeb1-29act5-ts	10	35	19	6	20	10
105	WT	0	77	0	1	16	6
	act5-ts	0	57	0	16	23	4
	yeb1Δ	1	69	1	1	21	7
	yeb1Δact5-ts	8	55	16	4	16	11
	yeb1-29act5-ts	9	48	18	4	13	8
120	WT	0	84	1	0	13	2
	act5-ts	0	63	0	14	19	3
	yeb1Δ	0	83	1	1	9	6
	yeb1Δact5-ts	15	51	21	2	8	3
	yeb1-29act5-ts	13	55	19	3	6	4
150	WT	0	89	1	0	6	4
	act5-ts	0	77	0	11	9	3
	yeb1Δ	0	87	1	1	8	3
	yeb1Δact5-ts	11	60	17	3	5	4
	yeb1-29act5-ts	12	63	16	3	3	3

Data in the last six columns indicate the percentages of different cell forms resulting over time in the indicated strains. A subset of these data is plotted in Fig. 2. WT, wild type; ts, temperature sensitive.