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. Author manuscript; available in PMC: 2008 Aug 1.
Published in final edited form as: J Immunol. 2007 Aug 1;179(3):1721–1729. doi: 10.4049/jimmunol.179.3.1721

FIGURE 3.

FIGURE 3

Phenotypic profile of MDCs and PDCs following rAd5 and rAd35 exposure. DC maturation was assessed after 24 h of exposure to rAd5 and rAd35. A, rAd35 induced full maturation of both DC subsets, whereas rAd5 induced only partial maturation. The histograms show expression levels of CD80 and CD40 after exposure to TLR7/8 ligand (blue), rAd35 (green), or rAd5 (red) compared with an unexposed control (filled gray). B and C, rAd35 doses of 5 (green), 1 (orange), or 0.5 (yellow) i.p./cell induced partial maturation, whereas a rAd5 dose of 200 (red) i.p. per cell induced no maturation. Unexposed MDCs (filled gray) were used as the negative undifferentiated control and TLR7/8 ligand-stimulated MDCs (blue) were used as a positive control for full maturation. D, Actual infection by the rAds of the PDCs but not the MDCs was associated with a more mature phenotype as visualized by higher cell surface expression of CD40 in the GFP+ PDCs vs the GFP− PDCs within the same culture. One representative donor of at least three is shown.

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