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. Author manuscript; available in PMC: 2008 Aug 1.
Published in final edited form as: J Immunol. 2007 Aug 1;179(3):1721–1729. doi: 10.4049/jimmunol.179.3.1721

FIGURE 5.

FIGURE 5

rAd induces polyfunctional memory CD8+ T cell responses. The DC subsets were exposed to rAd5-pp65 and rAd35-pp65 for 24 h at 1, 10, or 100 ip/cell before 6 h of coculture with autologous sorted T cells. A, Bars show total frequencies of responding (IFN-γ, TNF-α, IL-2, MIP-1β, CD107a expressing) pp65-specific CD8+ T cells. rAd35-pp65 exposure of either MDCs or PDCs induced a greater magnitude of pp65 responses than did rAd5-pp65 exposure. After exposure to rAd5-pp65 but not rAd35-pp65, MDCs were superior to PDCs at inducing CD8+ T cell responses. B, The portions of the pies represent the 31 combinations of responses and are shown grouped by number of functions: 5 (red); 4 (orange); 3 (yellow); 2 (green); and 1 (blue). MDCs exposed to either rAd5-pp65 or rAd35-pp65 induced a more polyfunctional CD8+ T cell response than PDCs, and one that resembles the response induced by the overlapping (OL) pp65 peptide stimulation. There was no difference between the qualities of the CD8+ response induced by rAd5-pp65 and rAd35-pp65.