Fig. 5.

Reovirus induced sensitization of OVCAR3 cells to TRAIL requires viral uncoating. OVCAR3 cells were infected with reovirus (MOI 10) in the presence or absence of ammonium chloride (AC, 10 mM). Twenty four h later the cells were treated with TRAIL (20 ng/ml). (A) 24 h following TRAIL treatment the percentage of cells with apoptotic nuclear morphology was determined. The graph shows the mean percentage of apoptotic cells from three independent experiments. Error bars indicate the standard error of the mean. Apoptosis was significantly reduced (P < 0.001,***) in reovirus-infected, TRAIL-treated cells in the presence of AC compared to that seen in reovirus-infected, TRAIL-treated cells without AC. (B) 6 h following TRAIL treatment cells were analyzed by western blot using antibodies directed against cleaved PARP and caspase 8. Anti-actin antibodies were used to control for protein loading