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Reovirus down-regulates cFLIP in infected OVCAR3 cells. OVCAR3 cells were infected with reovirus (MOI 10). After 18 h cells were then treated with TRAIL (20 ng/ml). Cells were harvested after a further 24 or 6 h and were analyzed by western blotting using antibodies directed against cFLIPL or cFLIPS, respectively. Anti-actin antibodies were used to control for protein loading
