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Depletion of NELF relieves promoter-proximal stalling, globally reducing PPEP. (a–c) Pol II (anti-Rpb3) ChIP-chip data from NELF-depleted (open circles) and mock-treated ChIP samples (blue squares) for genes with PPEP, ltd (CG8024-RC) (a) and wun2 (CG8805-RC) (b) as well as for a gene with more uniform Pol II signal, DNA Pol II-iota (CG7602-RB) (c). (d) Number of genes with PPEP observed on our partial ChIP-chip arrays in mock-treated and NELF-depleted Drosophila S2 cells.
