Table II. Sequence of the SMIT TonEs.
Sequencing of the small DNA fragments S14-c, S14-g, and S31-d (Fig. 2) revealed seven TonE sequences that conform to the consensus shown near the bottom. TonEB2 and TonEB3 overlap in reverse directions sharing 10 bp as follows: TGGAAAATTCCA, where TonEB2 is in bold letters and the complementary strand of TonEB3 is underlined. TonEp (p for proximal) is at the −331/−321 position. Taking active SMIT TonEs into consideration, an updated TonE consensus is derived (shown at the bottom). Y represents C or T; N represents A, G, C, or T.
Name | Location | Sequences | Remarks |
---|---|---|---|
TonEA | S14-c | TGGAAAACTAC | |
TonEB1 | S14-g | TGGAATTCTAT | Not active |
TonEB2 | S14-g | TGGAAAATTCC | Overlap with TonEB3 |
TonEB3 | S14-g | TGGAATTTTCC | Probably not active |
TonEC1 | S31-d | TGGAAAATTAC | Same as “hTonE” |
TonEC2 | S31-d | TGGAAAGTTAC | |
TonEC3 | S31-d | TGGAACTCCCT | Not active |
TonEp | −331/−321 | TGGAAAGTTCC | |
Consensus | YGGAANNNYNY | Ref. 22 | |
Updated consensus | TGGAAANNYNY |