Table 1.

Composition of the control diet and diet enriched with trans fatty acids according to AIN-93

	Diet (g/100 g)
Ingredient	Control	Trans
Casein*	20 (14)	20
L-cystine†	0.3 (0.18)	0.3
Cornstarch†	62 (71.1)	62
Soybean oil‡	8 (5)	1
Hydrogenated vegetable fat$	-	7
Butylhydroquinone†	0.0014 (0.0008)	0.0014 (0.0008)
Mineral mixture§	3.5	3.5
Vitamin mixture#	1.0	1.0
Cellulose†	5.0	5.0
Choline bitartrate†	0.25	0.25
Energy (kcal/g)	4.0 (3.8)	4.0 (3.8)

The first number refers to the growth diet (AIN-93G) and the number in parentheses refers to maintenance diet (AIN-93M) when its composition differed from that of the growth diet. *Casein was obtained from CoperLab, São Paulo, Brazil. ^†L-cystine, cornstarch, butylhydroquinone, cellulose and choline bitartrate were obtained from Viafarma, São Paulo, Brazil. ^‡Oil was supplied from soybean (Lisa/Ind. Brazil). ^$Hydrogenated vegetabke fat was supplied from Unilever, São Paulo, Brazil. ^£Mineral mix 9mg/kg diet): calcium, 5000; phosphorus, 1561; potassium, 3600; sodium, 1019; chloride, 1571; sulfur, 300; magnesium, 507; iron, 35; copper, 6.0; manganese, 10.0; zinc, 30.0; chromium, 1.0; iodine 0.2; selenium, 0.15; fluoride, 1.00; boron, 0.50; molybdenum, 0.15; silicon, 5.0; nickel, 0.5; lithium, 0.1; vanadium, 0.1 (AIN-93G mineral mix DYETS 210025, Dyets Inc., Bethlehem, PA, USA). ^#Vitamin mix (mg/kg diet): thiamin HCL, 6.0, riboflavin, 6.0; pyridoxine HCL 7.0; niacin, 30.0; calcium pantothenate, 16.0; folic acid, 2.0; biotin, 0.2; vitamin B12, 25.0; vitamin A palmitate 4000 IU; vitamin E acetate, 75; vitamin D3, 1000 IU; vitamin KI, 0.75. (AIN-93G, vitamin mix, DYETS 310025, Dyets Inc., Bethlehem, PA, USA)