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. Author manuscript; available in PMC: 2008 Oct 19.
Published in final edited form as: J Biol Chem. 2007 Aug 16;282(42):30763–30775. doi: 10.1074/jbc.M704655200

FIGURE 6. Catalytic activity of human PPIP5K1.

FIGURE 6

A, recombinant human PPIP5K1 (20 ng) was incubated at 37 °C in 100 μl of kinase assay buffer with PP-[3H]InsP5 for either 0 min (open circles)or 40 min(closed circles). Reactions were analyzed by Q100 HPLC. B, kinetic analysis of 5-PP-[3H]InsP5 phosphorylation using the indicated concentration of substrate. A representative experiment is shown, typical of three. The Km value = 0.34 μM, Vmax = 8.3 nmol/mg of protein/min (estimated by non-linear regression, using SigmaPlot, version 8.02).