. Author manuscript; available in PMC: 2009 Apr 25.

Published in final edited form as: Biochem Biophys Res Commun. 2007 Dec 3;369(1):124–134. doi: 10.1016/j.bbrc.2007.11.108

Table 1. Summary of actin-activated MgATPase activity and in vitro motility of HMM II-B.

Actin-activated MgATPase activities were measured at 25°C as described in Materials and methods. The values for V_max and K_ATPase are the mean and standard deviation from four different protein preparations. The IVM (in vitro motility) are from three preparations and the K⁺-EDTA ATPase values are the average of two preparations.

HMMs	Basal MgATPase	Actin-activated MgATPase			IVM	HMM K⁺-EDTA ATPase
		-MLCK	+MLCK
		V_max	V_max	K_ATPase
	s^-1	s^-1	s^-1	μM	μm/s	s^-1
II-B0	< 0.008	0.008±0.002	0.15±0.02	5.8±0.6	0.16±0.01	0.2
II-B2	< 0.006	0.006±0.003	0.01±0.003	2.8±0.8	NSM	0.2

II-B0, noninserted HMM II-B; II-B2, inserted HMM II-B with a 21-aa insert; NSM, no significant movement. Basal MgATPase is without actin, but with MLCK. [KCl] = 0 mM.