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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2008 Apr;49(4):1542–1552. doi: 10.1167/iovs.07-1300

FIGURE 1.

FIGURE 1

Activity of various chicken βB1-promoter fragments in lens cells at different stages of differentiation. (A) Transfection analysis of chicken βB1-promoter fragments in comparison with promoterless vector (=1) in N/N1003a cells, an established lens epithelial cell line. The differences between −126 and −152 (P < 0.01), −125 and −206 (P < 0.001), −245 and −282 (P < 0.049), and −402 and −432 (P < 0.001) are statistically significant, as determined by Student’s t-test. The differences between −206 and −245 (P < 0.15) and −282 and −402 (P < 0.32) are not statistically significant. (B) Western blot analysis of endogenous βB1-crystallin expression in CLAP cells from the time of isolation (0) and days 2, 4, and 6 culture in comparison with whole 18-day embryonic lenses (w) and isolated fiber cells (f). The apparent molecular weight of chicken βB1-crystallin is 35 kDa, as was previously reported.48 (C) Activity of βB1-promoter CAT plasmids in CLAP cells. The differences between −206 and −245 (P = 0.004), −245 and −282 (P = 0.04), −152 and −245 (P = 0.0006), and −245 and −432 (P = 0.0009) are statistically significant, as determined by Student’s t-test. The differences between −152 and −206 (P = 0.5) and −282 and −432 (P = 0.3) are not statistically significant. Results were combined from multiple transfection experiments. The total number of transfected plates for −152 is 17, for −206 is 9, for −245 is 16, for −282 is 6, and for −432 is 11. (D) Activity of βB1-promoter truncations in lenses of transgenic mice. The difference between −152/CAT and −245/CAT was significant (P = 0.01), and the difference between −245/CAT and −432/CAT was significant (P = 0.005). The −432/CAT and −152/CAT expression levels were previously determined to be significantly different (P = 0.0001). Absolute numbers reported here are different from those previously reported for the −152/CAT and −432/CAT transgenic mice8 because of differences in counting efficiency between the previous and current scintillation counter used in the laboratory. All error bars shown in this figure represent SD.