Table 1. Effects of GSHEE on E-C coupling.
Values represent mean ± SEM for n number of experiments. Parameters for the voltage dependence of Ca2+ conductance and Ca2+ transients were obtained as previously described (Chelu, 2006). Gmax, maximal L-channel conductance; (ΔF/F)max, maximal relative change in fluo-4 fluorescence; Vrev, L-channel reversal potential; VG1/2 and VF1/2, potential at which G and F are half-maximal, respectively; kG and kF, slope factors for IV and FV, respectively.
| Genotype | Gmax (nS/nF) | kG (mV) | VG1/2 (mV) | Vrev (mV) | (ΔF/F)max | kF (mV) | VF1/2 (mV) |
|---|---|---|---|---|---|---|---|
| RyR1wt/wt | 264 ± 13
n=6 |
6.4 ± 0.2
n=6 |
12.8 ± 0.8
n=6 |
76.8 ± 1.7
n=6 |
4.5 ± 0.4
n=6 |
5.4 ± 0.84
n=6 |
−16.9 ± 1.4
n=6 |
| RyR1Y522S/wt | 302 ± 22
n=7 |
4.7 ± 0.4
n=7 |
7.2 ± 0.7*
n=7 |
76.8 ± 2.9
n=7 |
2.9 ± 0.3*
n=7 |
8.5 ± 1.1
n=7 |
−29.0 ± 1.6*
n=7 |
| RyR1Y522S/wt + 5mM GSHEE | 266 ± 13
n=5 |
4.8 ± 0.2
n=5 |
1.3 ± 4.8
n=5 |
70.5 ± 4.6
n=5 |
3.9 ± 0.1
n=5 |
7.6 ± 1.6
n=5 |
−29.2 ± 1.4*
n=5 |
*
p<0.01 compared to RyR1wt/wt. Data are mean ± S.D.