IE1-pp65 ^ transfected autologous CD40-B cells expand CD4 and CD8 responses to the fusion protein and CD8 responses to pp65[495−503] peptide. CD3 Tcells from three CMV-seropositive/HLA-A*0201 ^ positive individuals (D1, D3,and D8) were stimulated weekly 3with IE1-pp65 fusion gene ^ transfected autologous CD40-B
× cells. Pre, at start of culture; post, after expansion in cultureT-cell responses were measured by IFN-γ ICC assay. Absolute numbers of responding CD4 and CD8 Tcells are shown before and after stimulation. All individuals showed 2 to 3 log-fold increases in target-specific CD4 and CD8 Tcells.