Table 2.
Characteristics of expanded T cells with repetitive stimulations by IE1-pp65 fusion gene — transfected autologous CD40-B cells in CMV-seropositive/HLA-A*0201 — positive healthy donors D1, D3, and D8
|
CD4/8 phenotype (%) |
IE1-pp65 fusion gene |
IE1-pp65 protein |
pp65[495−503] peptide |
CMV lysate, 10 μg |
Mock transfected |
CD40B alone |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Pre | Post | Pre | Post | Pre | Post | Pre | Post | Pre | Post | Pre | Post | Pre | Post | |
| D1 | ||||||||||||||
| CD4 | 58.5 | 9 | 0.04 | 0.3 | 0.1 | 2.44 | 0.02 | 0.08 | 0.07 | ND | 0.01 | 0.33 | 0.01 | 0.18 |
| CD8 | 39.5 | 87 | 5.4 | 18.5 | 0.07 | 0.48 | 0.38 | 3.93 | 0.03 | ND | 0.03 | 0.04 | 0.03 | 0.04 |
| D3 | ||||||||||||||
| CD4 | 59.5 | 18 | 0.07 | 2.9 | 0.3 | 5.0 | 0.04 | 0.27 | 0.38 | 1.2 | 0.04 | 0.38 | 0.03 | 0.15 |
| CD8 | 39 | 80 | 0.84 | 5.1 | 0.4 | 1.0 | 0.38 | 1.95 | 0.23 | 0.32 | 0.37 | 0.37 | 0.23 | 0.16 |
| D8 | ||||||||||||||
| CD4 | 40 | 6 | 0.1 | 1.71 | 0.06 | 1.24 | 0.03 | 0.14 | 0.14 | 1.32 | 0.02 | 0.35 | 0.03 | 0.14 |
| CD8 | 60 | 94 | 2.22 | 34.4 | 0.04 | 0.98 | 1.04 | 14.3 | 0.02 | 0.05 | 0.04 | 0.19 | 0.03 | 0.05 |
NOTE: Results show percentage of IFN-γ production after 3 weekly stimulations, determined by ICC assay for each stimulator cell type; phenotypic data is shown as percentage of cells expressing either CD4 or CD8. Significant differences are in bold.
Abbreviation: ND, not done.