Fig. 7.

ToxB and N17Rac1 each stimulate c-Jun activation and induction of Bim in CGNs: inhibition of ToxB-induced Bim expression by the pyridinyl imidazole, SB203580. (a) CGNs were incubated for up to 8 h with ToxB (40 ng/mL) and cell lysates were immunoblotted (IB) with a polyclonal antibody to c-Jun. The decreased mobility of c-Jun observed with increasing duration of ToxB exposure is indicative of activating phosphorylation. (b) CGNs were incubated for 6 h with either BSA/PBS vehicle (Veh) or ToxB ± SB203580 (SB; 20 μm). Following treatment, CGN lysates were immunoblotted for c-Jun. (c) CGNs were incubated for 6 h as described in (b). Cell lysates were then immunoblotted with a polyclonal antibody that detects an approximately 15-kDa isoform of Bim, Bim short (Bim_s). (d) CGNs were infected with either adenoviral GFP alone or in combination with N17Rac1. At 96 h post-infection, cell lysates were obtained and immunoblotted for c-Jun, Bim_s, and actin (as a loading control). NS, non-specific protein bands that are detected by the antibodies and are shown as loading controls.