Fig. 8.

The pyridinyl imidazole, SB203580, inhibits mitochondrial apoptotic signaling induced by ToxB in CGNs. (a) CGNs were incubated for 24 h with either vehicle (Veh; 2 μg/mL BSA in PBS) or ToxB (40 ng/mL) ± SB203580 (SB; 20 μm). Following incubation, cells were immunostained with a monoclonal antibody to the active conformation of Bax (clone 6A7) and nuclei were stained with DAPI. The arrows indicate cells that stained positively for active Bax. Scale bar, 10 μm. (b) CGNs were incubated exactly as described in (a) and either mitochondrial fractions were immunoblotted (IB) for cyt C or whole cell lysates were probed for active caspase-9 or -3. The blots shown are each indicative of data obtained in three separate experiments.