Figure 5.

Regulation of Gene Expression by E2 and Genistein in Cells Differentially Expressing ERα and ERβ

A, Transcript levels of estrogen-regulated genes (FOS, TFF1, and CASP7) were assessed by quantitative PCR after 4-h treatment of MCF-7 cells differentially expressing ERα and ERβ. To generate cells with ERα+ERβ or ERβ only vs. ERα only (parental MCF-7 cells), MCF-7 cells were infected with AdGal (control) or AdERβ for 24 h and subsequently transfected with either siCNTRL or siERα for an additional 48 h. Cells were then exposed to control vehicle (V), 6 nm E2, or 6 nm genistein (LG) for 4 h. Data represent average fold change ± sd of triplicate samples and is representative of three separate experiments.