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. 2008 Jan 31;22(5):1065–1077. doi: 10.1210/me.2007-0492

Figure 1.

Figure 1

The Expression of BRG1 But Not BAF57 Is Up-Regulated during Spontaneous Metamorphosis

RT-PCR was performed with primers specific for X. laevis BAF57 (A, C, and E) and BRG1 (B, D, and F) on total RNA isolated from whole animals, intestines, or tails of tadpoles at the indicated developmental stages, respectively (five animals for each group). The rpL8 gene served as an internal control in the same RT-PCR. Note that BRG1 mRNA levels increased at stages 62–64 in the tail as the tail resorbs (1.8-fold higher at stage 62 compared with stage 54 based on quantification using NIH ImageJ software). Higher levels of BRG1 mRNA were also observed in the intestine (1.7-fold higher at stage 62 compared with stage 54) or whole animals (1.4-fold higher at stage 62 compared with stage 54) at the climax (stage 62). BAF57 mRNA levels did not change significantly during development.