Figure 5.

Overexpression of BAF57 Enhances Transcriptional Activation by TR in a Dose-Dependent Manner

The mRNAs for TR/RXR (1.15 ng/oocyte) were injected into the cytoplasm of 20 oocytes together with F-BAF57 mRNA (1.15 or 2.3 ng/oocyte) as indicated. After 4 h of incubation, the TRE-Luc reporter vector together with the control Renilla luciferase plasmid were coinjected into the nucleus of the oocytes (lanes 1–7). The oocytes were incubated overnight with or without 50 nm T₃ as indicated. After incubation, the oocytes were lysed and subjected to dual-luciferase assays. The relative activity of the reporter vs. that of the control was plotted with the basal activity set to 100 (lane 1). The bars represent the means ± se of at least three independent experiments performed in triplicate (*, P < 0.05). In the lower panel, the same oocyte samples used in the luciferase assay (lanes 5–7) were subjected to Western blot analysis with the anti-Flag or TR antibody, confirming the expression of F-BAF57 and TR.