Figure 1.

PTHR1 Template and Sites of Substitution

Shown are amino acid residues A242, D241, K240, V239, F238 in TM2 and F447 in TM7 that were substituted with Cys in the present study. Also shown is the location of two FXa cleavage sites in ICL3. For detection by Western blot, a c-myc tag was inserted between residues 572 and 573 of the C-terminal intracellular domain. The endogenous cysteines are shown as open circles. aa, Amino acids.