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. Author manuscript; available in PMC: 2008 May 5.
Published in final edited form as: J Biol Chem. 2007 Mar 12;282(18):13532–13541. doi: 10.1074/jbc.M611295200

FIGURE 8. APE·Pol β·DNA ternary complex does not affect the APE 3′-5′ exonuclease activity.

FIGURE 8

Mismatched-nicked-THF flap substrate (10 nm) was incubated either with increasing concentrations of APE (5, 25, 50 nm) (lanes 2-6, respectively) or with 2.5 nm APE and increasing concentrations of Pol β (5-50 nm) (lanes 7-10, respectively) at 37 °C for 15 min as described under “Experimental Procedures.” Lane 1 was the reaction with only substrate DNA. The product and substrate were separated by denaturing polyacrylamide gel electrophoresis as described under “Experimental Procedures.” The substrate was made by annealing the 5′-end radiolabeled (asterisk) upstream primer and the downstream oligomer to the template. The substrate is schematically illustrated above the gel.