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. Author manuscript; available in PMC: 2008 May 5.
Published in final edited form as: Chem Res Toxicol. 2006 May;19(5):719–728. doi: 10.1021/tx0600245

Figure 1.

Figure 1

Detection of AP sites in PAH o-quinone-treated calf thymus DNA produced from heat depurination and depyrimidination for 2 h at 70 °C. (A) BP-7,8-dione, (B) 7,12-DMBA-3,4-dione and (C) BA-3,4-dione. See the Materials and methods for details. a, Basal level of AP sites in methoxyamine-treated calf thymus DNA; b, Heat-treated methoxyamine-treated calf thymus DNA (70 °C). A Wilcoxon two-sample exact test shows a marginally significant effect of temperature, i.e., more adducts were generated at 70 °C than 4 °C (p = 0.05). With the temperature set at 70 °C, Kruskal-Wallis tests show that BP-7,8-dione and 7,12-DMBA-3,4-dione had a significant effect on the quantity of adducts generated (p = 0.0084 and 0.0112, respectively) while BA-3,4-dione had no effect (p = 0.1304). Also, with the temperature set at 70 °C, linear regression analysis showed that the concentration of two quinones (BP-7,8-dione and 7,12-DMBA-3,4-dione) had a highly significant positive effect on the amount of depurinating adducts detected (p <0.0001 for both quinones; a positive effect indicates that the amount of the adducts increases as the concentration of each quinone increases).