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Activation of caspase 1-like and caspase 3-like proteases by Fas or reaper expression. Schneider cells (×) and their transformants harboring the expression plasmid for either FasC (○) or reaper (•) were treated with CuSO4 for the indicated periods of time. Cytosolic extracts were prepared from these cells, and caspase 1 and caspase 3 protease activities were determined using the fluorescent substrate MCA-YVADAPK(DNP) (A) or MCA-DEVDAPK(DNP) (B).
