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. Author manuscript; available in PMC: 2008 May 5.
Published in final edited form as: J Infect Dis. 2007 Mar 15;195(9):1365–1372. doi: 10.1086/513277

Figure 1.

Figure 1

Increased host plasminogen levels in the airways during staphylococcal pneumonia. A, Detection of plasminogen (mPLG) in bronchoalveolar lavage fluid (BALF) samples collected from C57BL/6 mice at 0.5 or 6 h after intranasal inoculation with either Staphylococcus aureus or PBS by modified ELISA using rabbit anti-mPLG antibody and goat anti-rabbit horseradish peroxidase–conjugated antibody. Absorbance was measured at 450 nm. Concentrations of mPLG were quantitated by comparison with a standard curve. B, BALF samples incubated in the absence or presence of recombinant staphylokinase (SAK; 10 μg/mL) for 15 min at 37°C. S-2251 chromogenic substrate (1 mmol/L) was added, and mPLG activation measured as absorbance at 405 nm for up to 24 h at 37°C. Data are the mean ± SD of triplicate values and are representative of 3 independent experiments. **P < .001, *P < .01, analysis of variance with Tukey’s post hoc test.