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. Author manuscript; available in PMC: 2008 May 5.
Published in final edited form as: J Infect Dis. 2007 Mar 15;195(9):1365–1372. doi: 10.1086/513277

Figure 2.

Figure 2

Up-regulation and processing of host cathelicidin in the airways during staphylococcal pneumonia. A, Detection of cathelicidin (mCRAMP) in bronchoalveolar lavage fluid (BALF) samples collected from C57BL/6 mice at 0.5 or 6 h after intranasal inoculation with either Staphylococcus aureus or PBS by modified ELISA using rabbit anti-mCRAMP antibody and goat anti-rabbit horseradish peroxidase–conjugated antibody. Absorbance was measured at 450 nm. Concentrations of mCRAMP were quantitated by comparison with a standard curve. Data are the mean ± SD of triplicate values and are representative of 3 independent experiments. **P < .001, analysis of variance with Tukey’s post hoc test. B, Western blot analysis of mCRAMP expression in BALF samples normalized for total protein content using the antibodies described in panel A. Synthetic mCRAMP peptide was used as a positive control.