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. Author manuscript; available in PMC: 2008 May 5.
Published in final edited form as: J Infect Dis. 2007 Mar 15;195(9):1365–1372. doi: 10.1086/513277

Figure 3.

Figure 3

Direct dose-dependent, binding of cathelicidin to staphylokinase (SAK). SAK (500 ng) and 1% bovine serum albumin (BSA) in carbonate buffer were immobilized in 96-well plates. Concentrations of cathelicidin (mCRAMP) ranging from 0.01 to 10 μg/mL were added to wells in the absence (A) or presence (B) of 5 μg/mL α-defensin. Bound mCRAMP was detected using anti-mCRAMP antibody and horseradish peroxidase–conjugated secondary antibody. Absorbance was measured at 450 nm. Specific binding was calculated as the difference in absorbance between SAK- and BSA-coated wells. Data are the mean ± SD of triplicate values and are representative of 3 independent experiments. *P < .01, Student’s t test.