Figure 10.

Inhibition of Ca²⁺ signaling decreases soft substrate–induced apoptosis. Normal cervical epithelial cells were cultured on collagen gel–coated dish (control) or on collagen gel (soft substrate) for 24 h in the absence or presence of various chemicals (1.5 mM EGTA, 20 μM 2-APB, 50 μM PD150606, PD151746, and PD145305) or STIM1 siRNA. Apoptotic analysis was assessed by flow cytometry of PI staining. Each column represents mean ± SEM from three different experiments. # p < 0.05; * p < 0.01, compared with soft substrate–induced apoptosis.