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. 2008 May;19(5):1883–1892. doi: 10.1091/mbc.E07-12-1230

Figure 1.

Figure 1.

Localization of DRhoGEF2, F-actin, and myosin II during segmental groove morphogenesis. (A and A′) Early stage 12: onset of groove invagination. DRhoGEF2 is enriched in groove founder cells marked by expression of Odd-skipped (Odd). (B and B′) Enlargement of A and A′, respectively. (C and C′) Early stage 12: polarization of F-actin at the posterior boundary of groove founder cells (marked by En-GFP in green) at the onset of groove invagination. (D and D′) Enlargement of C and C′, respectively. (E–E″) Early stage 12: apicolateral distribution of F-actin, myosin II, or DRhoGEF2 at the onset of groove formation. (F–F″) Mid-stage 12: as shallow furrows have formed, F-actin, myosin II, and DRhoGEF2 accumulate basally in epidermal cells (arrows). The epithelium now has a defined baseline. (G, H, and H′) Late stage 12: grooves reach their maximum depth as cells at the groove center elongate along their apical-basal axis (arrow in G). (I, J, and J′) Stage 13: onset of groove regression. F-actin, myosin II, and DRhoGEF2 accumulate at the apical cortex in cells posterior to segment boundaries (arrows). Grooves take on an asymmetrical appearance. (K, L, and L′) Stage 14: F-actin and myosin II form punctate condensations at the apical membrane. DRhoGEF2 remains uniform, and it is found in more apicolateral domains. (M, N, and N′) Stage 15: as grooves complete regression, epidermal cells take on a cuboidal shape. (O–O″) Stage 13: onset of groove regression. F-actin and DRhoGEF2 (brackets) accumulate in constricting cells posterior to grooves. (P–P″) Stage 15: DRhoGEF2 (brackets) does not colocalize with F-Actin punctas. (Q) Stage 14: DRhoGEF2 accumulates posterior to grooves in the lateral epidermis all the way to the dorsal leading edge. (R–R″) prd-Gal4>UAS-DRhoGEF2-RE; sqh-GFP embryo at stage 14. DRhoGEF2 overexpression recruits increased levels of myosin II to the cell membrane. E–P″ are ventrolateral views. A–D′ and Q–R″ are lateral views. Anterior is to the left and dorsal is up in all figures. Bars: 25 μm (A, A′, C, C′, and Q) and 10 μm (others).