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. 2008 May;19(5):1883–1892. doi: 10.1091/mbc.E07-12-1230

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© 2008 by The American Society for Cell Biology

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Figure 2. — Segmental groove formation requires DRhoGEF2 function. Embryos are stained for F-actin (red) and En (green) to mark segment boundaries. (A) DRhoGEF2^M-embryo at stage 13: most segmental grooves form in the correct position posterior to the en domains (arrowheads). (B) DRhoGEF2^MZ embryo at stage 13: segmental grooves do not form (arrowheads). (C) Ventral epidermis of DRhoGEF2^MZ embryo at stage 13: shallow indentations form in the epithelium (arrowheads). However, fully developed grooves do not form. (D) F-actin accumulates in irregular aggregates (arrowheads) in cells throughout the epithelium. Bars, 20 μm (A and B) and 10 μm (C and D).